Prokaryotic secretion pipeline

This pipeline includes different tools for secretome analysis and a set of Perl/Bioperl and R scripts has been set up. Prokaryotic analysis is presented under a one-step query interface, where an input FASTA file should be uploaded. User can select the cut-off scores for the processing tools (or default values). Optional tests are chosen and input file is submited.

* (G.O. enrichment test is not available for this analysis)

* Mandatory information

» Basic analysis

* Upload your FASTA file

* Select organism group

Enter SignalP cut-off probability:

Enter TargetP cut-off probability

Enter maximun transmembrane domains

» Optional analysis

Refine secretome prediction using PSORTb.
Enter your PSORTb cut-off score

Perform BLASTp orthology search
Enter BLASTp cut-off evalue

Perform Pfam domain screening

» Submission

Optional: insert an e-mail to receive your results link after completion.