Eukaryotic secretion pipeline

For a complete secretome analysis in eukaryotes, different tools for secretome analysis have been merged in a pipeline together with a set of Perl/Bioperl and R scripts. Tools for Eukaryotic analysis are presented under a one-step query interface, where users upload an input file in a format eligible between: Identifier lists (IDL) from different repositories, tables of counts (ToC), differential expresion tables (DEt) and FASTA files. Then selection of cut-off scores for the processing tools (or default values), and the optional tests to be performed (GO enrichment, Functional motifs and BLAST orthologous relations) can be chosen before data submission.

* Mandatory information

» Basic analysis

* Please, select the kind of input file

Please, select the organism

Please, select the kind of identifier in your ID list

Enter p-value cutt-off:

Enter minimun number of reads:

* Upload your file
(Maximum of 20.000 sequences or IDs allowed for each submission.)

Enter SignalP cut-off probability:

Enter TargetP cut-off probability

Enter maximun transmembrane domains

» Optional analysis

Refine secretome prediction using WoLF PSORT.
Enter your WoLF PSORT cut-off score

Select kingdom

Perform BLASTp orthology search
Enter BLASTp cut-off evalue

Perform Pfam domain screening

Perform GO enrichment analysis
Enter the treshold:

» Submission

Optional: insert an e-mail to receive your results link after completion.